miRNAgFree is a novel microRNA prediction approach based on biogenesis features, such as the known 5’ homogeneity, and duplex mean free energies which does not require a genome assembly. We found that, in general, biogenesis related parameters are far more discriminative than duplex related structural parameters.

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miRNAgFree can currenlty be downloaded as a standalone tool (download here).

For a more detailed description of the software implementation, example command lines, modes and parameters descriptions please check miRNAgFree manual.